A Distinct Human CD4+T Cell Subset That Secretes CXCL13 in Rheumatoid Synovium

作者：Kobayashi Shio; Murata Koichi; Shibuya Hideyuki; Morita Mami; Ishikawa Masahiro; Furu Moritoshi; Ito Hiromu; Ito Juichi; Matsuda Shuichi; Watanabe Takeshi; Yoshitomi Hiroyuki^*

来源：Arthritis and Rheumatism, 2013, 65(12): 3063-3072.

DOI：10.1002/art.38173

ObjectiveA subset of CD4+ T cells in the synovium of patients with rheumatoid arthritis (RA) produce CXCL13, a chemokine that is crucial for the formation of germinal centers. This study was undertaken to determine the relevance of this population to known subsets of T helper cells and to proinflammatory cytokines, and how these cells are generated.
MethodsThe expression of Th markers and CXCL13 by CD4+ T cells in RA synovium and the involvement of proinflammatory cytokines in CXCL13 production were assessed. We also investigated whether CXCL13+CD4+ T cells could be newly induced.
ResultsCXCL13+CD4+ T cells in RA synovium were negative for interferon- (IFN), interleukin-4 (IL-4), IL-17, FoxP3, and CXCR5 and expressed low levels of inducible T cell costimulator, indicating that this population is a distinct human CD4 subset. T cell receptor (TCR) stimulation of CD4+ T cells, obtained from RA synovium with low expression of CXCL13, promptly induced CXCL13 production and addition of proinflammatory cytokines supported the long-term production of CXCL13. These findings indicate that CXCL13-producing CD4+ T cells can be in a memory state ready to be reactivated upon TCR stimulation and that proinflammatory cytokines are involved in persistent CXCL13 production. TCR stimulation of CD4+ T cells from the blood of healthy volunteers, together with proinflammatory cytokine supplementation, induced a population that produced CXCL13, but not IFN. Synovial T cells recruited CXCR5+ cells in a CXCL13-dependent manner.
ConclusionCXCL13-producing CD4+ T cells induced in RA synovium may play a role in the recruitment of CXCR5+ cells, such as B cells and circulating follicular helper T cells, and in ectopic lymphoid neogenesis at sites of inflammation.

• 出版日期2013-12