Melamine-Induced Urolithiasis in a Drosophila Model

作者：Chen Wen Chi; Lin Wei Yong; Chen Huey Yi; Chang Chiao Hui; Tsai Fuu Jen; Man Kee Ming; Shen Jui Lung; Chen Yung Hsiang^*

来源：Journal of Agricultural and Food Chemistry, 2012, 60(10): 2753-2757.

DOI：10.1021/jf204647p

• Drosophila melanogaster
• melamine
• urolithiasis
• potassium citrate

Melamine-tainted food can induce renal stones in both humans and animals. We have previously reported a novel Drosophila model for the study of renal stone disease. In addition to hyperoxaluria-causing agents, we also tested herein the effect of melamine on crystal formation in Drosophila. The results indicate that administration of melamine alone caused crystal formation in a dose-dependent manner. The crystals also appeared after ingestion of melamine for 3 weeks in the Malpighian tubules of Drosophila when viewed with polarized light. Administration of potassium citrate (K citrate) was found to significantly ameliorate the melamine-induced reduction of lifespan. However, administration of K citrate failed to reduce the quantity of crystals. Because calcium oxalate is not the major crystal induced by melamine, the predominant components of melamine-induced crystals and the potential crystal inhibitors warrant further investigation.

• 出版日期2012-3-14
• 单位中国医科大学