Dietary n-3 long-chain polyunsaturated fatty acids modify phosphoenolpyruvate carboxylcinase activity and lipid synthesis from glucose in adipose tissue of rats fed a high-sucrose diet

作者：Londero Lisiane G; Rieger Debora K; Hansen Fernanda; Silveira Simone L; Martins Tiago L; Lulhier Francisco; da Silva Roselis S; Souza Diogo O; Perry Marcos L S; de Assis Adriano M^*

来源：Cell Biochemistry and Function, 2013, 31(8): 636-642.

DOI：10.1002/cbf.2947

• n-3 long-chain polyunsaturated fatty acids
• adipose tissue
• high-sucrose diet
• insulin resistance
• phosphoenolpyruvate carboxykinase

Long-chain polyunsaturated n-3 fatty acids (n-3 LCPUFAs) have hypolipidemic effects and modulate intermediary metabolism to prevent or reverse insulin resistance in a way that is not completely elucidated. Here, effects of these fatty acids on the lipid profile, phosphoenolpyruvate carboxykinase (PEPCK) activity, lipid synthesis from glucose in epididymal adipose tissue (Ep-AT) and liver were investigated. Male rats were fed a high-sucrose diet (SU diet), containing either sunflower oil or a mixture of sunflower and fish oil (SU-FO diet), and the control group was fed a standard diet. After 13weeks, liver, adipose tissue and blood were harvested and analysed. The dietary n-3 LCPUFAs prevented sucrose-induced increase in adiposity and serum free fat acids, serum and hepatic triacylglycerol and insulin levels. Furthermore, these n-3 LCPUFAs decreased lipid synthesis from glucose and increased PEPCK activity in the Ep-AT of rats fed the SU-FO diet compared to those fed the SU diet, besides reducing lipid synthesis from glucose in hepatic tissue. Thus, the inclusion of n-3 LCPUFAs in the diet may be beneficial for the prevention or attenuation of dyslipidemia and insulin resistance, and for reducing the risk of related chronic diseases.

• 出版日期2013-12