DETECTION OF APOPTOSIS IN HUMAN AND RAT OVARIAN FOLLICLES

作者：PIQUETTE GN; TILLY JL; PRICHARD LE; SIMON C; POLAN ML

来源：Journal of the Society for Gynecologic Investigation, 1994, 1(4): 297-301.

• APOPTOSIS
• HUMAN OVARY
• RAT OVARIAN FOLLICLES
• LUTEINIZATION

Objective: The purpose of this study was to determine whether cells acquired from individual human preovulatory follicles undergo apoptosis (physiologic cell death) and, if so, to correlate the degree of apoptosis with characteristics of the follicles or the oocytes derived from the follicles.
Methods: We devised a sensitive nonradioactive method for detecting apoptotic DNA fragmentation in small numbers of cells derived from rat atretic follicles and follicular aspirates of patients undergoing assisted reproductive technologies.
Results: Using this method, apoptotic DNA was detected in rat atretic follicles, with optimal detection at 10-100 ng. Furthermore, apoptotic DNA was detected in some, but not all individual human follicular aspirates from several patients, and was found in follicles that produced oocytes that fertilized and developed into embryos.
Conclusion: Apoptosis occurs in cells from human ovarian preovulatory follicles and may be a normal physiologic process of the follicle during luteinization.

• 出版日期1994-12