Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli

作者：Kitamatsu Mizuki^*; Kurami Shunsuke; Ohtsuki Takashi; Sisido Masahiko

来源：Bioorganic & Medicinal Chemistry Letters, 2011, 21(1): 225-227.

DOI：10.1016/j.bmcl.2010.11.034

• Peptide nucleic acid
• Cell-penetrating peptide
• Antisense effect

To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-L-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures.

• 出版日期2011-1