科研之友
微信
新浪微博
Facebook
分享链接
摘要
Large conductance Ca2+-activated K+ channels (BKCa) contribute to negative feedback regulation of smooth muscle cell (SMC) tone. However, the effects of aging on BKCa function are unclear. We tested the hypothesis that aging alters SMC BKCa function in superior epigastric arteries (SEAs) by using perforated patch recording of enzymatically isolated SMCs from 3- to 4-mo-old male C57BL/6 mice (Young) and 24- to 26-moold male C57BL/6 mice (Old). SMC capacitance from Young (15.7 +/- 0.4 pF; n = 110) was less than Old (17.9 +/- 0.5 pF; n = 104) (P < 0.05). SMCs displayed spontaneous transient outward currents (STOCs) at membrane potentials more positive than -30 mV; depolarization increased STOC amplitude and frequency (P < 0.05; n = 19-24). STOC frequency in Young (2.2 +/- 0.6 Hz) was less than Old (4.2 +/- 0.7 Hz) at -10 mV (P < 0.05, n = 27-30), with no difference in amplitude (1.0 +/- 0.1 vs. 0.9 +/- 0.1 pA/pF, respectively). At + 30 mV, STOC amplitude in Young (3.2 +/- 0.3 pA/pF) was less than Old (5.0 +/- 0.5 pA/pF; P < 0.05, n = 61-67) with no difference in frequency (3.9 +/- 0.4 vs. 3.2 +/- 0.3 Hz, respectively). BKCa blockers (1 mu M paxilline, 100 nM iberiotoxin, 1 mM tetraethylammonium) or a ryanodine receptor antagonist (100 mu M tetracaine) inhibited STOCs (n >= 6; P < 0.05 each). Western blots revealed increased expression of BKCa alpha-subunit protein in Old. Pressure myography revealed no effect of age on SEA maximal diameter, myogenic tone, or paxilline-induced constriction (n = 10-12; P > 0.05). Enhanced functional expression of SMC BKCa-dependent STOCs in Old may represent an adaptation of resistance arteries to maintain functional integrity.
- 出版日期2014-6
