Automating the application of smart materials for protein crystallization

作者:Khurshid Sahir; Govada Lata; El Sharif Hazim F; Reddy Subrayal M; Chayen Naomi E*
来源:ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2015, 71(3): 534-540.
DOI:10.1107/S1399004714027643

摘要

The fabrication and validation of the first semi-liquid nonprotein nucleating agent to be administered automatically to crystallization trials is reported. This research builds upon prior demonstration of the suitability of molecularly imprinted polymers (MIPs; known as 'smart materials') for inducing protein crystal growth. Modified MIPs of altered texture suitable for high-throughput trials are demonstrated to improve crystal quality and to increase the probability of success when screening for suitable crystallization conditions. The application of these materials is simple, time-efficient and will provide a potent tool for structural biologists embarking on crystallization trials.

  • 出版日期2015-3