p62/SQSTM1 in autophagic clearance of a non-ubiquitylated substrate

作者：Watanabe Yoshihisa; Tanaka Masaki^*

来源：Journal of Cell Science, 2011, 124(16): 2692-2701.

DOI：10.1242/jcs.081232

• Non-ubiquitylated substrate
• Aggresome
• HDAC6
• p62/SQSTM1
• Autophagy

Proteolytic systems and the aggresome pathway contribute to preventing accumulation of cytotoxic aggregation-prone proteins. Although polyubiquitylation is usually required for degradation or aggresome formation, several substrates are processed independently of ubiquitin through a poorly understood mechanism. Here, we found that p62/SQSTM1, a multifunctional adaptor protein, was involved in the selective autophagic clearance of a non-ubiquitylated substrate, namely an aggregation-prone isoform of STAT5A (STAT5A_Delta E18). By using a cell line that stably expressed STAT5A_Delta E18, we investigated the properties of its aggregation and degradation. We found that STAT5A_Delta E18 formed non-ubiquitylated aggresomes and/or aggregates by impairment of proteasome functioning or autophagy. Transport of these aggregates to the perinuclear region was inhibited by trichostatin A or tubacin, inhibitors of histone deacetylase (HDAC), indicating that the non-ubiquitylated aggregates of STAT5A_Delta E18 were sequestered into aggresomes in an HDAC6-dependent manner. Moreover, p62 was bound to STAT5A_Delta E18 through its PB1 domain, and the oligomerization of p62 was required for this interaction. In p62-knockdown experiments, we found that p62 was required for autophagic clearance of STAT5A_Delta E18 but not for its aggregate formation, suggesting that the binding of p62 to non-ubiquitylated substrates might trigger their autophagic clearance.

• 出版日期2011-8-15