A novel mechanism for momordin Ic-induced HepG2 apoptosis: involvement of PI3K-and MAPK-dependent PPARγ activation

作者：Wang, Jing; Yuan, Li; Xiao, Haifang; Wang, Chan; Xiao, Chunxia; Wang, Yutang; Liu, Xuebo^*

来源：Food & Function, 2014, 5(5): 859-868.

DOI：10.1039/c3fo60558b

• LUNG-CARCINOMA CELLS
• GASTRIC-CANCER CELLS
• OXIDATIVE STRESS
• TRANSCRIPTION FACTOR
• COX-2 INHIBITION
• EXPRESSION
• PATHWAY
• FOXO
• ROLES
• ACID

Momordin Ic is a natural triterpenoid saponin found in various Chinese and Japanese natural medicines such as the fruit of Kochia scoparia (L.) Schrad. Momordin Ic has been previously demonstrated to induce HepG2 cell apoptosis in a ROS-mediated PI3K and MAPK pathway-dependent manner. In the present study, the underlying mechanisms of PI3K and MAPK pathway-mediated PPAR gamma, and PGC-1 alpha co-regulator activation, as well as the effects of downstream proteins, COX-2 and FoxO4, on cell apoptosis were investigated. The results demonstrated that momordin Ic activated PPAR gamma and inhibited COX-2. PGC-1 alpha and FoxO4 expressions were increased by the PI3K or MAPK pathways. Furthermore, PPAR gamma inhibition decreased p-p38 and FoxO4 expression, and restored COX-2 expression. ROS inhibition exerted little effect on PPAR gamma, COX-2 and FoxO4 expression but affected PGC-1 alpha expression. These results revealed the involvement of PI3K and MAPK-dependent PPAR gamma activation in momordin Ic-induced apoptosis, providing more detailed information underlying the pro-apoptotic mechanism of momordin Ic in HepG2 cell apoptosis.

• 出版日期2014-5
• 单位西北农林科技大学