A multiplatform real-time polymerase chain reaction detection assay for Vibrio cholerae

作者：Koskela Katja A; Matero Pirjo; Blatny Janet M; Fykse Else M; Olsen Jaran Strand; Nuotio Lasse O; Nikkari Simo^*

来源：Diagnostic Microbiology and Infectious Disease, 2009, 65(3): 339-344.

DOI：10.1016/j.diagmicrobio.2009.07.009

• Real-time PCR
• Vibrio cholera
• toxR
• ctxA
• Field detection

We report a multiplatform real-time polymerase chain reaction methodology based on genes encoding for the regulatory toxR activator and enterotoxin A protein to determine enterotoxigenic Vibrio cholerae types from other vibrios. This assay, which was successfully validated on a collection of 87 bacterial strains, including 63 representatives of V cholerae and 8 noncholera vibrios provides a rapid tool for detection and identification of cholera.

• 出版日期2009-11