Degradation of high-molecular-weight hyaluronan by hydrogen peroxide in the presence of cupric ions

作者：Soltes L^*; Brezova V; Stankovska M; Kogan G; Gemeiner P

来源：Carbohydrate Research, 2006, 341(5): 639-644.

DOI：10.1016/j.carres.2006.01.014

• hyaluronan degradation
• rotational viscometry
• EPR spectroscopy
• spin-trapping technique
• DMPO

Dynamic viscosity (eta) of the high-molecular-weight hyaluronan (HA) solution was measured by a Brookfield rotational viscometer equipped with a Teflon CLIP and spindle of coaxial cylindrical geometry. The decrease of eta of the HA solution, indicating degradation of the biopolymer, was induced by it system containing H2O2 alone or H2O2 plus CuCl2. The reaction system H2O2 plus CuCl2 as investigated by EPR spin-trapping technique revealed the formation of a four-line EPR signal characteristic of a DMPO-OH spin adduct. Thus, hydroxyl radicals are implicated in degradation of high-molecular-weight HA by the system containing H2O2 and CuCl2.

• 出版日期2006-4-10