Analysis of the Kanamycin in Raw Milk Using the Suspension Array

作者：Wang Yanfei; Zou Mingqiang^*; Han Yi; Zhang Fan; Li Jinfeng; Zhu Xiaoyan

来源：Journal of Chemistry, 2013, 2013: 784543.

DOI：10.1155/2013/784543

With the monoclonal antibody against kanamycin being prepared successfully, a bead-based indirect competitive fluorescent immunoassay was developed to detect kanamycin in milk. The fact that there was no significant cross-reaction with other aminoglycoside antibiotics implied that the monoclonal antibody was highly specific for kanamycin. The limit of detection (LOD) and the 50% inhibition concentration (IC50) in raw milk were 3.2 ng/mL and 52.5 ng/mL, respectively. Using the method developed in this study, the kanamycin concentrations were monitored in raw milk after the intramuscular administration of kanamycin in sick cows. Compared to the conventional enzyme-linked immunosorbent assay (ELISA), the method using the suspension array system was more sensitive. The results obtained in the present study showed a good correlation with that of the ELISA.

• 出版日期2013
• 单位中国科学院