Angiotensin I-converting enzyme inhibitory peptides in red-mold rice made by Monascus purpureus

作者：Kuba Megumi; Tanaka Kumi; Sesoko Masayasu; Inoue Fumihide; Yasuda Masaaki^*

来源：Process Biochemistry, 2009, 44(10): 1139-1143.

DOI：10.1016/j.procbio.2009.06.007

• Red-mold rice
• Monascus purpureus
• Angiotensin I-converting enzyme inhibitor
• Hypertension
• Bioactive peptide
• Tofuyo

The ACE inhibitory activity in red-mold rice extracts, prepared from 24 strains of the genus Monascus, was measured. The most effective strain for ACE inhibition was Monascus purpureus IFO 4489 (IC(50) = 0.71 mg/ml). Although the antihypertensive substance gamma-amino butyric acid was detected in the red-mold rice (85.2 mg/kg), it did not contribute to ACE inhibition. Four ACE inhibitory peptides were isolated from the extract and identified as Ile-Tyr (IC(50) = 4.0 mu M), Val-Val-Tyr (22.0 mu M), Val-Phe (49.7 mu M) and Val-Trp (3.1 mu M) by protein sequencing. The ACE inhibitory activity of these peptides was almost completely preserved after successive in vitro digestion by pepsin, chymotrypsin and trypsin. These results suggest that red-mold rice made by M. purpureus could be useful in alleviating hypertension.

• 出版日期2009-10