Adsorption of Human Serum Albumin (HSA) on a mixed-mode adsorbent: equilibrium and kinetics

作者：Gomes Pedro Ferreira; Loureiro Jos Miguel; Rodrigues Alirio E^*

来源：Adsorption, 2017, 23(4): 491-505.

DOI：10.1007/s10450-017-9861-x

• Human Serum Albumin
• Mixed-mode chromatography
• Adsorption Isotherms
• Adsorption Kinetics
• Batch adsorption
• Packed bed adsorption

The adsorption equilibrium and kinetics of Human Serum albumin (HSA) onto a novel high particle density multimodal adsorbent were studied by batch adsorption experiments. MabDirect MM, from Upfront Chromatography A/S, is an agarose-tungsten carbide composite adsorbent with an anionic mixed mode ligand. The effects of ionic strength (by addition of salt) and of pH are assessed. Langmuir isotherms parameters are obtained along with pore diffusivity values by fitting the batch experiments using a pore diffusion model. Under the studied conditions for pH 5.0, without salt, it was obtained the largest adsorption capacity (36.0 +/- 3.5 mg center dot g(adsorbent,dry) (-1)) a typical behavior for a mixed mode adsorbent. Effective pore diffusivity (D-pe) was estimated as (2.2 +/- 0.1) x 10(-6) cm(2)center dot min(-1) for this condition. Additionally, the lowest adsorption capacity (8.6 +/- 2.1 mg center dot g(adsorbent,dry) (-1)) and an estimation of D-pe equal to (2.4 +/- 0.6) x 10(-6) cm(2)center dot min(-1) were obtained for pH 7.0 without salt. An alternative method of D-pe estimation is used and validate the results obtained by simulations. Furthermore, several experiments were carried out in a fixed bed column with the aim to understand the kinetics and hydrodynamics, and to validate the batch adsorption results. For a feed concentration of +/- 0.92 g center dot L-1, pH 5.0, without salt, for cleaned and fresh adsorbent, fixed bed dynamic binding capacities of 13.84 and 14.37 mg center dot g(adsorbent,dry) (-1) were obtained, respectively, representing near 50% of saturation capacity.

• 出版日期2017-5