Assessing corneal biomechanics with Brillouin spectro-microscopy

作者:Lepert Guillaume; Gouveia Ricardo M; Connon Che J; Paterson Carl*
来源:Faraday Discussions, 2016, 187: 415-428.
DOI:10.1039/c5fd00152h

摘要

A new Brillouin spectro-microscope was designed and built to investigate the mechanical properties of bovine and human corneas. This instrument integrates a single-stage virtually imaged phased array spectrometer with a novel adaptive-optics interferometric filter to achieve unprecedented rejection of the elastic background signal. As a result, highly-resolved, reproducible data from both thin and thick collagen-based materials were obtained. In particular, this technique is capable of rigorously measuring the relative stiffness of different areas of human corneas, thus providing a true non-contact method to characterise the fundamental mechanical features of both live and fixed biological tissue samples.

  • 出版日期2016