Detection of Deoxynivalenol Using Fluorescence Excitation-Emission Matrix

作者：Fujita Kaori; Tsuta Mizuki^*; Kokawa Mito; Sugiyama Junichi

来源：Food and Bioprocess Technology, 2010, 3(6): 922-927.

DOI：10.1007/s11947-010-0397-2

• Deoxynivalenol
• Excitation-emission matrix
• Fluorescence
• Fusarium
• Mycotoxin
• Principal component analysis

Deoxynivalenol (DON) is an acutely toxic mycotoxin produced in wheat and other cereal grains when exposed to fungi such as Fusarium spp. In this study, DON aqueous samples at seven concentration levels were examined using the excitation-emission matrix (EEM). EEM is a graph composed of an excitation wavelength axis, an emission wavelength axis, and a fluorescence intensity axis. It is acquired by measuring the fluorescence intensity of a sample at consecutive excitation and emission wavelengths. The EEM of DON solution showed fluorescent peaks, which were nonexistent in that of water, particularly in the excitation (Ex) and emission (Em) wavelength ranges: Ex 200-240 nm/Em 300 nm and Ex 250-300 nm/Em 400-500 nm. Since the EEM is a multidimensional data composed of many wavelength conditions (667 in this case), principal component analysis was applied to reduce the data to lower dimensions. By plotting the scores of the first and second principal components, it was shown that DON could be discriminated from water and the concentration of DON in the aqueous solution could be judged.

• 出版日期2010-12