A PROTEOMIC BASED ASSESSMENT ON CHANGES IN MYOFIBRILLAR PROTEINS OF GOAT LONGISSIMUS MUSCLE AS AFFECTED BY HEAT TREATMENTS

作者：Sarah S A^*; Karsani S A; Amin I; Mokhtar N F K; Sazili A Q

来源：Journal of Animal and Plant Sciences, 2014, 24(2): 406-412.

• goat meat
• heat treatments
• myofibrillar protein
• 2-dimensional electrophoresis
• MALDI-TOF/TOF

The present study examined the effect of different heat treatments; (1) chilled, (2) boiled at 100 degrees C for 30 min, and (3) autoclaved at 121 degrees C at 15 psi for 20 min, on the expression of goat skeletal muscle proteins using two-dimensional gel electrophoresis. The molecular weight (MW) and isoelectric point (pI) of heat stable proteins were characterised followed by identification of the proteins by MALDI-TOF/TOF mass spectrometry. There were 153 protein spots obtained in the boiled samples, while only 46 protein spots were observed in the autoclaved samples. Thirteen spots that exhibited high intensity of protein were chosen from the autoclaved sample for MALDI-TOF/TOF mass spectrometry analysis. The putative heat stable proteins identified were myosin light chain (MLC), actin, tropomyosin (TPM), troponin T (TnT), myoglobin, and creatine kinase. The Proc-GLM analysis revealed that the heat treatments have resulted in significant differences in spot intensities of actin, troponin T (TnT), myoglobin, and creatine kinase with no significant changes noted in other proteins.

• 出版日期2014