Application of poly(styrene-divinylbenzene) resin to the reversed phase high performance liquid chromatography for the determination of thymosin alpha

作者：Shao Cheng Wei; Wei Rong Qing; Zhang Ting Ting; Liu Xiao Ning^*

来源：Chinese Journal of Analytical Chemistry, 2007, 35(10): 1491-1494.

• poly(styrene-divinylbenzene)
• chromatographic column
• reversed phase high performance liquid chromatography
• thymosin alpha(1)

A reversed phase high performance liquid chromatographic( RP-HPLC) method with polystyrene-divinylbenzene (PS-DVB) resin(MKF-RP-MH) was developed for the determination of thymosin alpha(1). In the method, thymosin alpha(1) was chromatographically analyzed with a mobile phase A:0.01 mol/L phosphate buffer solution( pH = 7.0 or 8.0) and B: acetonitrile, linear gradient elution condition: 0-20 min, 0%-10% acetonitrile, 20-40 min, 10%-30% acetonitrile; column temperature was set at 30 degrees C; flow rate was 0.8 mL/min; The detection wavelength was 214 nm. The standard sample and two kinds of thymosin a, samples were analysised under the optimum chromatographic condition. The calibration curve was in good linearity within the range of 0.05-4.0 g/L with the regression equation of C = 0.0099A-0.0798 (r = 0.9994). The maximum amount of thymosin alpha(1) on MKF-RP-MH column was 120 mu g (6 g/L).

• 出版日期2007-10
• 单位南京工业大学