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Adaptive divergence between incipient species of Anopheles gambiae increases resistance to Plasmodium
White, Bradley J
Lawniczak, Mara K N
Cheng, Changde
Coulibaly, Mamadou B
Wilson, Michael D
Sagnon, N'Fale
Costantini, Carlo
Simard, Frederic
Christophides, George K
Besansky, Nora J
Proceedings of the National Academy of Sciences of the United States of America, 108(1), pp 244-249, 2011-1-4
Summary
The African malaria mosquito Anopheles gambiae is diversifying into ecotypes known as M and S forms. This process is thought to be promoted by adaptation to different larval habitats, but its genetic underpinnings remain elusive. To identify candidate targets of divergent natural selection in M and S, we performed genome-wide scanning in paired population samples from Mali, followed by resequencing and genotyping from five locations in West, Central, and East Africa. Genome scans revealed a significant peak of M-S divergence on chromosome 3L, overlapping five known or suspected immune response genes. Resequencing implicated a selective target at or near the TEP1 gene, whose complement C3-like product has antiparasitic and antibacterial activity. Sequencing and allele-specific genotyping showed that an allelic variant of TEP1 has been swept to fixation in M samples from Mali and Burkina Faso and is spreading into neighboring Ghana, but is absent from M sampled in Cameroon, and from all sampled S populations. Sequence comparison demonstrates that this allele is related to, but distinct from, TEP1 alleles of known resistance phenotype. Experimental parasite infections of advanced mosquito intercrosses demonstrated a strong association between this TEP1 variant and resistance to both rodent malaria and the native human malaria parasite Plasmodium falciparum. Although malaria parasites may not be direct agents of pathogen-mediated selection at TEP1 in nature-where larvae may be the more vulnerable life stage-the process of adaptive divergence between M and S has potential consequences for malaria transmission.
Keywords
ecological speciation; malaria vector; population genomics; thioester immune gene
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