DNase 1 Retains Endodeoxyribonuclease Activity Following Gold Nanocluster Synthesis

作者：West Abby L^*; Griep Mark H; Cole Daniel P; Karna Shashi P

来源：Analytical Chemistry, 2014, 86(15): 7377-7382.

DOI：10.1021/ac5005794

Here we present the synthesis of the enzyme DNase 1 stabilized gold nanoclusters (DNase 1:AuNCs) with core size consisting of either 8 or 25 atoms. The DNase 1:Au8NCs exhibit blue fluorescence whereas the DNase 1:Au25NCs are red emitting. In addition to the intense fluorescence emission, the synthesized DNase 1:AuNC hybrid retains the native functionality of the protein, allowing simultaneous detection and digestion of DNA with a detection limit of 2 mu g/mL. The DNase 1:AuNCs could be conveniently employed as efficient and fast sensors to augment the current time-consuming DNA contamination analysis techniques.

• 出版日期2014-8-5