Application of Nano-Baskets and Hyphenated Emulsion Liquid Membrane-Nuclear Magnetic Resonance (ELM-NMR) in Metabolomics of Rat Serum

作者：Mokhtari Bahram; Pourabdollah Kobra^*

来源：Journal of the Chemical Society of Pakistan, 2012, 34(6): 1482-1490.

A novel approach for metabolite extraction and fingerprinting was introduced based upon application of nano-baskets of calix[ 4] arene in emulsion liquid membrane-nuclear magnetic resonance (ELM-NMR) technique. The objective of this method is introducing novel applications of nano-baskets, optimizing the fingerprints, minimizing the metabolic variation from analysis, increasing the likelihood differences, and obtaining the maximum extraction yield. Low molecular weight metabolites in rat serum were recovered by ELMs using eight nano-baskets of calixarene as emulsifier and carrier. The yields of ELMs were optimized by the method of one-at-a-time. According to NMR data, the maximum metabolic variation was achieved using scaffold 4 (4 wt%), n-decane membrane, stirring rate of 300 rpm, treat and phase ratios of 0.3 and 0.8, respectively. Referring to NMR spectrum, the results revealed that other calixarenes tend to extract non-specific macromolecules and the repeatability of fingerprints was more for 7-mediated ELM and was minimum for 3-mediated ELM. The yield of extractions was obtained to be higher for n-decane and lower for carbon tetrachloride. Using different membrane types, the fingerprints by chlorinated liquid membranes were more repeatable than toluene or n-decane.

• 出版日期2012-12