Comparison of four current 25-hydroxyvitamin D assays

作者：Moon Hee Won; Cho Jin Hee; Hur Mina^*; Song Junghan; Oh Gwi Young; Park Chul Min; Yun Yeo Min; Kim Jin Q

来源：Clinical Biochemistry, 2012, 45(4-5): 326-330.

DOI：10.1016/j.clinbiochem.2011.12.025

• Vitamin D
• Assay
• Correlation
• Agreement
• Standardization

Objectives: The performance of recently developed vitamin D total assays (ADVIA Centaur and Elecsys) was compared to that of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and LIASON 25-OH Vitamin D total assays. %26lt;br%26gt;Design and methods: A total of 157 clinical samples and standard reference material (SRM) 972 were analyzed. %26lt;br%26gt;Results: The correlations of LC-MS/MS with the three immunoassays were acceptable. However, compared to LC-MS/MS, LIAISON and ADVIA Centaur showed negative bias, and Elecsys showed positive bias. There was a lack of agreement among the four methods with only LC-MS/MS results close to the certified values of SRM 972. The prevalence of vitamin D insufficiency (%26lt;50 nmol/L) was higher with ADVIA Centaur (51.6%) and LIAISON (52.2%) and lower with Elecsys (37.6%), compared with that of LC-MS/MS (44.6%). %26lt;br%26gt;Conclusion: The new, automated total vitamin D assays show acceptable correlation with LC-MS/MS, and could be used in routine laboratories. However, standardization of vitamin D assays and consideration of assay-specific decision limits should be addressed.

• 出版日期2012-3