科研之友
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摘要
Fusarium wilt is an important root disease of cucumber throughout the world. There are no accurate simple sequence repeat (SSR) markers for use in molecular breeding of fusarium wilt resistance and no studies on chromosomal mapping of the resistance in cucumber. In this paper, a set of 148 F9 recombinant inbred lines derived from the cross 9110Gt x 9930 and a total of 2,416 pairs of SSR primers were used to study the inheritance of fusarium wilt resistance and to detect quantitative trait loci (QTLs) conferring the resistance in cucumber. Genetic analysis indicated that the resistance to fusarium wilt in 9110Gt was quantitative. One major QTL, Foc2.1, was screened in the years 2007, 2009 and 2012. It accounted for phenotypic variances of 64.2, 32.2 and 38.8 % with logarithm of odds scores of 32.78, 12.51 and 15.15 in the three years, respectively. The major QTL was placed in the region of SSR03084-SSR17631 within a genetic distance of 2.4 cM on chromosome 2. The physical length of the genomic region harboring Foc2.1 was 751.6 kb and there were seven predicted nucleotide binding site resistance genes. The validation of SSR17631 linked to Foc2.1 was tested using 46 diverse germplasms. SSR17631 had an accuracy rate of 87.88 % for selecting resistant materials and it could be used to screen cucumber resources with fusarium wilt resistance in molecular marker-assisted selection breeding. The major QTL identified in this paper will help to understand the genetic basis of fusarium wilt resistance. The present study has provided a firm step for fine mapping and gene cloning of fusarium wilt resistance in cucumber in the future.
- 出版日期2014-12
- 单位中国农业科学院
