Antibacterial activity tannin-rich fraction from leaves of Anacardium humile

作者：Bueno Ferreira Peracio Rafael; Oliveira Mendes Cintia Sorandra; Rodrigues Cinthia Gracielly; Moura Rocha Juliane Caroline; Royo Vanessa de Andrade; Valerio Henrique Maia; de Oliveira Dario Alves

来源：Ciencia Rural, 2012, 42(10): 1861-1864.

DOI：10.1590/S0103-84782012005000080

• phenolic
• cajui
• antibacterial
• MIC
• MBC
• Sephadex LH-20

In vitro tests conducted with extracts rich in tannins have identified several biological activities of this class of substance. Thus, this paper intends to evaluate the antibacterial activity of tannin-rich fraction obtained from leaf extracts of Anacardium humile A.St.-Hil. Extracts of A. humile leaves in 70% acetone were semi-purified with ethyl acetate and butanol. We quantified the total tannins of the semi-purified fractions, of the crude extract and of aqueous residues and then performed tests of the antibacterial activity of the tannins against Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. All positive extracts underwent tannin isolation using a Sephadex LH-20 column. The tannins isolated from the samples were quantified and tested for the minimal inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). The tannins from crude extracts, semi-purifications and residues showed inhibition of S. aureus growth with MIC=500 mu g mL(-1). All tannin fractions showed MIC against all strains and MBC, except against E. faecalis. The tannin fractions from Anacardium humile have antibacterial activity against Staphylococcus aureus, Pseudomononas aeruginosa and Enterococcus faecalis and, therefore, may be promising for future synthesis of new antibacterial agents.

• 出版日期2012-10