A novel multifunctional nanocomposite C225-conjugated Fe3O4/Ag enhances the sensitivity of nasopharyngeal carcinoma cells to radiotherapy

作者：Zhao Di; Sun Xinchen^*; Tong Jinglong; Ma Jun; Bu Xiaodong; Xu Ruizhi; Fan Renhua

来源：Acta Biochimica et Biophysica Sinica, 2012, 44(8): 678-684.

DOI：10.1093/abbs/gms051

• radiotherapy
• radiosensitivity
• molecular targeted therapy
• nasopharyngeal carcinoma cells

Radiotherapy is the major treatment for nasopharyngeal carcinoma, a malignant tumor of epithelial origin. In this process, a tracer with high sensitivity is pivotal for diagnostic imaging in radiotherapy. Here, we designed a novel multifunctional magnetic silver nanocomposite, Fe3O4/Ag conjugated to an epidermal growth factor receptor-specific antibody (C225), which can be potentially used for synchronous cancer therapy and diagnosis via magnetic resonance imaging. Characteristics of Fe3O4/Ag/C225 were determined by transmission electron microscopy, energy dispersive X-ray spectroscopy, ultraviolet spectra, and dynamic light scattering. The results demonstrated that Fe3O4/Ag/C225 nanoparticles were spherical and dispersed well in water. The activity of C225 was preserved approximate to 80 in the Fe3O4/Ag/C225 nanoparticles. Futhermore, we tested the cytotoxicity and radiosensitivity of the nanocomposite for human nasopharyngeal carcinoma cell lines (CNEs) in vitro. MTT analysis revealed that Fe3O4/Ag/C225 could inhibit the proliferation of CNEs in a dose- and time-dependent manner. The clonogenic assay indicated that Fe3O4/Ag/C225 combined with X-ray treatment could increase the sensitivity of CNEs to irradiation. In a summary, the novel multifunctional nanocomposite Fe3O4/Ag/C225 might be a potential radiosensitizer for treating malign tumors in the clinic.

• 出版日期2012-8
• 单位东南大学