Antiperoxidation activity of triterpenoids from rhizome of Anemone raddeana

作者：Chen Xin; Lu Jincai^*; He Wenfei; Chi Haidong; Yamashita Koichi; Manabe Masanobu; Kodama Hiroyuki

来源：Fitoterapia, 2009, 80(2): 105-111.

DOI：10.1016/j.fitote.2008.11.003

• Anemone raddeana Regel
• Triterpenoids
• Human neutrophils
• Superoxide
• Tyrosine phosphorylation
• Cytosolic compounds

Four triterpenoid compounds hederacolchiside E (1), hederasaponin B (2), raddeanoside 20 (3) and raddeanoside 21 (4) were isolated from ethanol extracts of rhizome of Anemone raddeana Regel. The effects of these triterpenoids on superoxide generation, tyrosyl phosphorylation of proteins and translocation of cytosolic compounds, such as p47(phox), p67(phox) and Rac to the cell membrane in human neutrophils was investigated. The superoxide generation induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) was slightly suppressed by hederasaponin B, raddeanoside 20 and raddeanoside 21 in a concentration dependent manner. The superoxide generation induced by arachidonic acid (AA) was suppressed by hederasaponin B and raddeanoside 21 significantly. fMLP- and AA-induced tyrosyl phosphorylation and translocation of the cytosolic proteins: p47(phox), p67(phox), and Rac to the cell membrane were suppressed in parallel with the suppression of stimulus-induced superoxide generation.

• 出版日期2009-3
• 单位沈阳药科大学; 温州医科大学