科研之友
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摘要
A rapid and simple enrichment system was developed on microfluidic chip which was integrated with on-line complexing and fluorescence detection. Microparticles of ion-exchange resin were trapped into the microchannel by a fabricated weir-structure in the end of the microchannel to construct a microsolid-phase extraction (mu-SPE) device. Some commonly existing metal ions in environment were served as models to evaluate the performance of the proposed microdevice, in combination with on-line derivatization with 8-hydroxyquinolin-5-sulfonic acid (HQS) and fluorescence detection. The concentration and pH value of HQS solution were optimized for metal-HQS fluorescent derivatization. The parameters, which affected the efficiency of the developed method, including composition and concentration of eluent, pH value and the flow rate of HQS solution and elution, were also investigated. Under the optimal conditions, Ca2+, Mg2+, Zn2+ and Pb2+ were successfully determined by the mu-SPE device on-chip. The experimental enrichment factors for Ca2+, Mg2+, Zn2+ and Pb2+ were up to 520, 565, 578 and 487 folds, respectively.
- 出版日期2013-11-15
- 单位北京市理化分析测试中心; 清华大学
