Bone marrow mesenchymal stem cells with Nogo-66 receptor gene silencing for repair of spinal cord injury

作者：Li Zhiyuan^*; Zhang Zhanxiu; Zhao Lili; Li Hui; Wang Suxia; Shen Yong

来源：Neural Regeneration Research, 2014, 9(8): 806-814.

DOI：10.4103/1673-5374.131595

• nerve regeneration
• spinal cord injury
• bone marrow mesenchymal stem cells
• Nogo-66 receptor
• RNA interference
• horseradish peroxidase
• BrdU
• gene silencing
• neural regeneration

We hypothesized that RNA interference to silence Nogo-66 receptor gene expression in bone marrow mesenchymal stem cells before transplantation might further improve neurological function in rats with spinal cord transection injury. After 2 weeks, the number of neurons and BrdU-positive cells in the Nogo-66 receptor gene silencing group was higher than in the bone marrow mesenchymal stem cell group, and significantly greater compared with the model group. After 4 weeks, behavioral performance was significantly enhanced in the model group. After 8 weeks, the number of horseradish peroxidase-labeled nerve fibers was higher in the Nogo66 receptor gene silencing group than in the bone marrow mesenchymal stem cell group, and significantly higher than in the model group. The newly formed nerve fibers and myelinated ner ve fibers were detectable in the central transverse plane section in the bone marrow mesenchymal stem cell group and in the Nogo-66 receptor gene silencing group.

• 出版日期2014-4
• 单位河北医科大学