Differential response of macrophages to core-shell Fe3O4@Au nanoparticles and nanostars

作者：Xia Wei; Song Hyon Min; Wei Qingshan; Wei Alexander^*

来源：Nanoscale, 2012, 4(22): 7143-7148.

DOI：10.1039/c2nr32070c

Murine RAW 264.7 cells were exposed to spheroidal core-shell Fe3O4@Au nanoparticles (SCS-NPs, ca. 34 nm) or nanostars (NSTs, ca. 100 nm) in the presence of bovine serum albumin, with variable effects observed after macrophagocytosis. Uptake of SCS-NPs caused macrophages to adopt a rounded, amoeboid form, accompanied by an increase in surface detachment. In contrast, the uptake of multibranched NSTs did not induce gross changes in macrophage shape or adhesion, but correlated instead with cell enlargement and signatures of macrophage activation such as TNF-alpha and ROS. MTT assays indicate a low cytotoxic response to either SCS-NPs or NSTs despite differences in macrophage behavior. These observations show that differences in NP size and shape are sufficient to produce diverse responses in macrophages following uptake.

• 出版日期2012