Aqueous extract of tomato seeds attenuates rotenone-induced oxidative stress and neurotoxicity in Drosophila melanogaster

作者：Krishna Gokul; Muralidhara

来源：Journal of the Science of Food and Agriculture, 2016, 96(5): 1745-1755.

DOI：10.1002/jsfa.7281

<jats:title>Abstract</jats:title><jats:sec><jats:title>BACKGROUND</jats:title><jats:p>Tomato seeds, a major by‐product from the food processing industry, constitute a rich source of bioactives and a large population consumes tomato (either in raw or cooked form). In the present study, initially we assessed the antioxidant activity of aqueous extract of tomato seeds (<jats:styled-content style="fixed-case">TSE</jats:styled-content>) in selected chemical systems and further explored the neuroprotective effects of <jats:styled-content style="fixed-case">TSE</jats:styled-content> utilising the rotenone (<jats:styled-content style="fixed-case">ROT</jats:styled-content>) model of neurotoxicity in <jats:italic>Drosophila</jats:italic>.</jats:p></jats:sec><jats:sec><jats:title>RESULTS</jats:title><jats:p>Adult male flies (Oregon K) were fed <jats:styled-content style="fixed-case">TSE</jats:styled-content>‐enriched medium (0.1–0.2%) with or without <jats:styled-content style="fixed-case">ROT</jats:styled-content> (500 µmol L<jats:sup>−1</jats:sup>) for 7 days. The propensity of <jats:styled-content style="fixed-case">TSE</jats:styled-content> to protect flies against <jats:styled-content style="fixed-case">ROT</jats:styled-content>‐induced lethality, locomotor phenotype, oxidative stress and neurotoxicity was investigated. <jats:styled-content style="fixed-case">TSE</jats:styled-content> offered marked protection against <jats:styled-content style="fixed-case">ROT</jats:styled-content>‐induced mortality, while survivors exhibited improved locomotor phenotype. <jats:styled-content style="fixed-case">TSE</jats:styled-content> significantly attenuated <jats:styled-content style="fixed-case">ROT</jats:styled-content>‐induced oxidative stress, mitochondrial dysfunctions, protein carbonyls content, restored the cholinergic function and dopamine levels.</jats:p></jats:sec><jats:sec><jats:title>CONCLUSION</jats:title><jats:p>We hypothesise that the efficacy of tomato seed extract to attenuate <jats:styled-content style="fixed-case">ROT</jats:styled-content>‐mediated neurotoxicity may be largely related to the combined antioxidant activity of bioactives resulting in abrogation of oxidative stress and mitochondrial dysfunction. More importantly, our approach provides an experimental paradigm to rapidly assess the potential neuroprotective effects of common dietary components employing <jats:italic>Drosophila</jats:italic>, since it corroborates previous evidence in a mouse model.

• 出版日期2016-3-30