科研之友
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摘要
An efficient protocol which involved mechanical destruction of cell walls by freeze thawing and removal of polysaccharide by cetyltrimethylammonium bromide precipitation is described for the rapid isolation of high molecular weight (greater than or equal to 50 kb) DNA from the Spirulina platensis. The DNA yield ranged 1.0-1.54 mu g/mg dry wt with the purity measured at A(260/280) of 1.77-1.82. The DNA preparation was suited as substrate for restriction and PCR.
- 出版日期1997-9
- 单位中山大学
