MicroRNAs control transcription factor NF-kB (p65) expression in human ovarian cells

作者：Sirotkin Alexander V^*; Alexa Richard; Kisova Gabriela; Harrath Abdel Halim; Alwasel Saleh; Ovcharenko Dmitriy; Mlyncek Milos

来源：Functional & Integrative Genomics, 2015, 15(3): 271-275.

DOI：10.1007/s10142-014-0413-0

• miRNAs
• NF-kB (p65)
• Ovary
• Reproductive medicine

MicroRNAs (miRNAs) are known to influence ovarian cell proliferation, apoptosis and hormone release, but it remains unknown whether miRNAs affect ovarian functions via transcription factors. We examined the effect of miRNAs on nuclear factor-kappaB (NF-kB) (p65) expression in human ovarian luteinized granulosa cells. We transfected cultured primary human ovarian luteinized granulosa cells with 80 different constructs encoding human pre-miRNAs and then evaluated NF-kB (p65) expression (percentage of cells containing p65) by immunocytochemistry. We found that 21 of the constructs stimulated NF-kB (p65) expression and 18 of the constructs inhibited NF-kB (p65) expression. This is the first direct demonstration that miRNAs affect NF-kB (p65) expression and the first genome-scale miRNA screen to identify upregulation and downregulation of NF-kB accumulation by miRNAs in the ovary. Novel miRNAs that affect the NF-kB signalling pathway could be useful for the control of NF-kB-dependent reproductive processes and the treatment of NF-kB-dependent reproductive disorders.

• 出版日期2015-5