Alginate-Chitosan versus Chitosan-Alginate Multi-Layered Assembled Systems: In Situ Comparative QCM-D Study

作者：Ramasamy T G; Haidar Z S^*

来源：Journal of Biomaterials and Tissue Engineering, 2012, 2(1): 83-88.

DOI：10.1166/jbt.2012.1029

• Alginate
• Chitosan
• Film
• Layer-by-Layer
• Polyelectrolytes
• Thickness
• Visco-Elastic

For nano-scale surface modification and biofabrication purposes, it is necessary to first understand the variations in adsorption behavior of certain carbopolymers. Assembled polyelectrolyte multi-layers (PEMs) composed of two naturally-derived polyelectrolytes; chitosan (CHI) and alginate (ALG) were alternatively deposited via the layer-by-layer technique in reversed order on planar gold (Au) surfaces and monitored in situ by means of quartz crystal microbalance with dissipation (QCM-D) in real-time. QCM-D revealed that [ALG-CHI](10) flilms were compact yet soft with thickness in the range of 240 nm compared to a more open [CHI-ALG](10) system with 91 nm. Such differences in visco-elastic properties of PEMs were the result of different growth regimes employed. To the best of our knowledge, this is the first QCM-D demonstration of the difference in multi-layer structure by alternate assembly of ALG and CHI on a flat surface. Findings suggest that more robust PEMs could be produced when ALG is the prime base layer.

• 出版日期2012-3