Activation of IKK/NF-kappa B provokes renal inflammatory responses in guanylyl cyclase/natriuretic peptide receptor-A gene-knockout mice

作者：Das Subhankar; Periyasamy Ramu; Pandey Kailash N^*

来源：Physiological Genomics, 2012, 44(7): 430-442.

DOI：10.1152/physiolgenomics.00147.2011

• atrial natriuretic peptide receptor-A
• gene targeting
• proinflammatory cytokines
• nuclear factor-kappa B
• renal fibrosis
• inhibitory kappa B kinase

First published February 7, 2012; doi: 10.1152/physiolgenomics. The present study was aimed at determining the consequences of the disruption of guanylyl cyclase/natriuretic peptide receptor-A (GC-A/NPRA) gene (Npr1) on proinflammatory responses of nuclear factor kappa B, inhibitory kappa B kinase, and inhibitory kappa B alpha (NF-kappa B, IKK, I kappa B alpha) in the kidneys of mutant mice. The results showed that the disruption of Npr1 enhanced the renal NF-kappa B binding activity by 3.8-fold in 0-copy (-/-) mice compared with 2-copy (+/+) mice. In parallel, IKK activity and I kappa B alpha protein phosphorylation were increased by 8- and 11-fold, respectively, in the kidneys of 0-copy mice compared with wild-type mice. Interestingly, I kappa B alpha was reduced by 80% and the expression of proinflammatory cytokines and renal fibrosis were significantly enhanced in 0-copy mice than 2-copy mice. Treatment of 0-copy mice with NF-kappa B inhibitors andrographolide, pyrrolidine dithiocarbamate, and etanercept showed a substantial reduction in renal fibrosis, attenuation of proinflammatory cytokines gene expression, and significantly reduced IKK activity and IkB alpha phosphorylation. These findings indicate that the systemic disruption of Npr1 activates the renal NF-kappa B pathways in 0-copy mice, which transactivates the expression of various proinflammatory cytokines to initiate renal remodeling; however, inhibition of NF-kappa B pathway repairs the abnormal renal pathology in mutant mice.

• 出版日期2012-4