High-Speed Tracking of Murine Cardiac Stem Cells by Harmonic Nanodoublers

作者：Magouroux Thibaud; Extermann Jerome; Hoffmann Pernilla; Mugnier Yannick; Le Dantec Ronan; Jaconi Marisa E^*; Kasparian Christelle; Ciepielewski Daniel; Bonacina Luigi; Wolf Jean Pierre

来源：Small, 2012, 8(17): 2752-2756.

DOI：10.1002/smll.201200366

• harmonic nanoparticles
• nonlinear microscopy
• embryonic stem cells
• cell tracking
• regenerative medicine

Potassium niobate nonlinear nanoparticles are used for the first time to monitor the evolution of embryonic stem cells (ESC) by second harmonic microscopy. These particles feature the complete absence of photo-bleaching and unlimited excitation wavelength flexibility. The potential of this approach is made evident for tissue-regeneration studies and applications, by capturing a high-speed movie of ESC-derived cardiomyocytes autonomously beating within a cluster. Time-resolved data are analyzed to retrieve 3D information of the contraction pattern at the cellular level.

• 出版日期2012-9-10