Development of microsatellite markers from an ectomycorrhizal fungus, Laccaria amethystina, by a dual-suppression-PCR technique

作者：Wadud MA^*; Lian CL; Nara K; Ishida TA; Hogetsu T

来源：Molecular Ecology Notes, 2006, 6(1): 130-132.

DOI：10.1111/j.1471-8286.2005.01163.x

• ectomycorrhizae
• Laccaria amethystina
• population genetics
• SSR

Laccaria amethystina is a major early successional ectomycorrhizal fungus. We isolated 10 polymorphic loci from L. amethystina using a dual-suppression-PCR (polymerase chain reaction) technique. These loci provided microsatellite markers with polymorphism of two to 10 alleles per locus. The observed and expected heterozygosities ranged from 0.136 to 0.545 and 0.206 to 0.877, respectively.

• 出版日期2006-3