Novel antihelmintic activity of tinidazole coordination compounds. Relevance of the metal ion and structural properties

作者：Alfaro Fuentes Israel; Castro Ramirez Rodrigo; Ortiz Pastrana Naytze; Maria Medina Guerrero Rosa; Catherinne Soler Jimenez Lilia; Martinez Rodriguez Irma; Betancourt Lozano Miguel; Ibarra Castro Leonardo; Barba Behrens Norah^*; Josefina Fajer Avila Emma

来源：Journal of Inorganic Biochemistry, 2017, 176: 159-167.

DOI：10.1016/j.jinorgbio.2017.08.013

• Tinidazole coordination compounds
• Snappers
• Antihelmintic activity
• Metal ions
• Ectoparasites

The in vitro and in vivo antihelmintic activity of cobalt(II), copper(II) and zinc(II) coordination compounds of tinidazole (tnz) were investigated in cultivated spotted rose snapper, infested with dactylogyrid monogeneans. The tinidazole coordination compounds [Co(tnz)(2)Cl-2], [Co(tnz)(2)Br-2], [Cu(tnz)(2)Cl-2], [Cu(tnz)(2)Br-2], [Zn (tnz)(2)Cl-2] and [Zn(tnz)(2)Br-2) were synthesized and spectroscopically characterized. Their molecular structures were determined by their single crystal X-ray diffraction. The metal ions presented distorted tetrahedral geo-metries, with an intramolecular bifurcated lone pair S=O center dot center dot center dot pi, from the sulfone group with the imidazolic ring, which contributed to the stability of the compounds in solid state and in solution. Adults of dactylogyrids were exposed in vitro to tinidazole and its coordination compounds. The effective median concentrations of copper(II) coordination compounds were lower than those of cobalt(II) and zinc(II), tnz showed no activity. In vivo oral intubation tests were carried out with [Cu(tnz)(2)Br-2], [Zn(tnz)(2)Br-2] and tnz on snappers infected with dactylogyrids, where the copper(II) compound showed better activity. The absorption and distribution assessment for the [Cu(tnz)(2)Br-2], showed that copper concentrations in liver were significantly higher than in blood and gills, indicating bioaccumulation in this organ. In vivo baths of [Cu(tnz)(2)Br-2] at 25 mg/L showed an effective (95% at 8 h) antihelmintic effect, while [Zn(tnz)(2)Br-2] had low antihelmintic efficacy. This study indicates that [Cu (tnz)(2)Br-2] has an effective antihelmintic activity towards dactylogyrids monogeneans affecting cultivated spotted red snapper.

• 出版日期2017-11