A Tobacco Etch Virus Protease with Increased Substrate Tolerance at the P1%26apos; position

作者：Renicke Christian; Spadaccini Roberta; Taxis Christof^*

来源：PLos One, 2013, 8(6): e67915.

DOI：10.1371/journal.pone.0067915

Site-specific proteases are important tools for in vitro and in vivo cleavage of proteins. They are widely used for diverse applications, like protein purification, assessment of protein-protein interactions or regulation of protein localization, abundance or activity. Here, we report the development of a procedure to select protease variants with altered specificity based on the well-established Saccharomyces cerevisiae adenine auxotrophy-dependent red/white colony assay. We applied this method on the tobacco etch virus (TEV) protease to obtain a protease variant with altered substrate specificity at the P1%26apos; Position. In vivo experiments with tester substrates showed that the mutated TEV protease still efficiently recognizes the sequence ENLYFQ, but has almost lost all bias for the amino acid at the P1%26apos; Position. Thus, we generated a site-specific protease for synthetic approaches requiring in vivo generation of proteins or peptides with a specific N-terminal amino acid.

• 出版日期2013-6-24