A Novel Osteoblast/Osteocyte Selection Method in Primary Isolated Chick Bone Cells by Atomic Force Microscopy

作者：Han Sung Woong; Tamaki Takato; Adachi Taiji^*

来源：Journal of Nanoscience and Nanotechnology, 2015, 15(5): 3923-3927.

DOI：10.1166/jnn.2015.9536

• Osteoblast/Osteocyte Selection
• Atomic Force Microscopy
• Force Spectroscopy
• Membrane Protein Detection
• Cell Elastic Modulus

Selection of bone cells, particularly osteoblasts and osteocytes, for analysis of cellular processes and differentiation is a very important issue because bone remodeling is a highly complex and harmonized process, which includes molecular and cellular interactions and communications. In this study, we introduce a novel osteoblast and osteocyte selection method that uses atomic force microscopy and OB7.3, an antibody of Phex, which is a specific protein marker expressed on the surface of osteocytes. The elasticity and Phex expression levels were simultaneously detected by force spectroscopy using the OB7.3-modified atomic force microscopy probe on the bone cell surface. The elastic modulus was different between osteoblasts and osteocytes. Phex expression level was analyzed by the distribution of Phex-OB7.3 rupturing.