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摘要
This study develops a gel permeation chromatography-high performance liquid chromatography-fluorescence detection (GPC-HPLC-FLD) method for determination of benzo(a) pyrene and aflatoxins (B1, B2, G1, G2) in vegetable oil. In the method, sample is extracted with ethyl acetate/cyclohexane (1 : 1, v/v), and cleaned up with the GPC. The separation of target compounds is performed on a Extend C-18 column (4.6 x 250 mm, 5 mu m) at 25 degrees C with methanol and 10 mmol L-1 ammonium acetate solution as mobile phase with gradient elution at a flow rate of 1.0 mL min(-1). The injection volume was 10 mu L, detection wavelengths were set at 360 nm (lambda(ex)) and 440 nm (lambda(em)) at 0-23 min, and 380 nm (lambda(ex)) and 408 nm (lambda(em)) at 23-35 min using FLD. The detection limits of benzo(a) pyrene and aflatoxins (B1, B2, G1, G2) were 0.5, 1.0, 1.0, 1.0 and 1.0 mu g kg(-1), respectively. The linear detection ranges of benzo(a) pyrene and aflatoxins (B1, B2, G1, G2) are 0.5-25.0 mu g kg(-1), 1.0-30.0 mu g kg(-1), 1.0-10.0 mu g kg(-1), 1.0-30.0 mu g kg(-1), 1.0-10.0 mu g kg(-1) with correlation coefficients (R-2) of 0.9998, 0.9999, 0.9997, 0.9998, 0.9996, respectively. Recovery rates in vegetable oil spiked with target compounds are in the range of 82.6-101.6%, with the relative standard deviation of 4.85-9.84%. The real sample tests show that this simple and accurate method can be used for determination of benzo(a) pyrene and aflatoxins (B1, B2, G1, G2) in vegetable oil.
- 出版日期2014
- 单位北京市海淀区产品质量监督检验所
