Analgesic effect of TAK-242 on neuropathic pain in rats

作者：Zhao, Yang; Xin, Yan; Gao, Jie; Teng, Ru-Yang; Chu, Hai-Chen^*

来源：International Journal of Clinical and Experimental Medicine, 2015, 8(7): 11202-11207.

• Neuropathic pain
• TAK-242

Background: The current study investigated the analgesic effect of the Toll-like receptor 4 (TLR4) specific antagonist TAK-242 on neuropathic pain in rats and its underlying mechanism. Methods: A total of 132 adult Sprague-Dawley (SD) rats were randomly divided into four groups: the sham operation group, the neuropathic pain model group, the TAK-242 low-dose treatment group, and the TAK-242 high-dose treatment group. The heat pain and mechanic pain thresholds of rats were detected on preoperative day 1 and postoperative days 1, 3, 7, and 10. The expression levels of I kappa B alpha, p65, IL-1 beta, and TNF-alpha in the spinal cord dorsal horn were detected on postoperative day 7 in one group of rats. Results: Compared with rats in the sham operation group, the heat pain and mechanic pain thresholds of the rats in the neuropathic pain model group significantly decreased; their expression levels of p65, IL-1 beta, and TNF-alpha significantly increased; and their expression level of IkB alpha significantly decreased. Compared with the neuropathic pain group, high doses of TAK-242 significantly inhibited the expression of p65, IL-1 beta, and TNF-alpha; significantly increased the expression level of IkBa; and upregulated the heat pain and mechanic pain thresholds. Conclusion: TAK-242 might improve neuropathic pain through downregulation of the NF-kappa B pathway.

• 出版日期2015
• 单位青岛大学; 青岛市市立医院