Diagnosis of Enteric Fever by Widal and Two Dot-enzyme Immunoassay: Utility and Difficulties

作者：Hosamani Manjunath A^*; Patil Asha B; Nadagir Shobha D; Madhusudhan N S; Sambrani Pramod

来源：Journal of Pure and Applied Microbiology, 2013, 7(3): 2379-2383.

• Enteric fever
• Widal test
• Typhidot test

Enteric fever is an important cause of mortality and morbidity in developing and under developed countries. The present study was undertaken to detect antibodies to salmonella using serological tests i.e. Two dot-enzyme immunoassay (Typhidot) and Widal test and correlation of the results. Consecutive 100 blood samples collected from clinically suspected cases of enteric fever were processed using standard methods. The samples were subjected to blood culture, widal test & typhidot test. Blood culture was positive in 7(7%), Widal test positive in 20(20%) and Typhidot in 28(28%). The sensitivity, specificity, PPV and NPV for Typhidot and Widal when compared with blood culture as gold standard were 100%, 77.41%, 25%, 100% and 85.71%, 84.95%, 30%,98.75% respectively. Typhidot test being rapid, inexpensive, easy to perform and highly sensitive test, has an advantage over conventional methods like blood culture and widal test in diagnosis of enteric fever caused by salmonella typhi. Typhidot is also more useful in detection of enteric fever in the 1st and 2nd week of infection.

• 出版日期2013-9
• 单位河北医科大学