Automated glycan assembly using the Glyconeer 2.1 synthesizer

作者：Hahm Heung Sik; Schlegel Mark K; Hurevich Mattan; Eller Steffen; Schuhmacher Frank; Hofmann Johanna; Pagel Kevin; Seeberger Peter H^*

来源：Proceedings of the National Academy of Sciences, 2017, 114(17): E3385-E3389.

DOI：10.1073/pnas.1700141114

• oligosaccharide synthesis
• automated synthesis
• glycochemistry
• glycobiology
• Glyconeer

Reliable and rapid access to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological research and medical practice. Similarly, the procurement of defined glycans is key to establishing structure-activity relationships and thereby progress in the glycosciences. Here, we describe the rapid assembly of oligosaccharides using the commercially available Glyconeer 2.1 automated glycan synthesizer, monosaccharide building blocks, and a linker-functionalized polystyrene solid support. Purification and quality-control protocols for the oligosaccharide products have been standardized. Synthetic glycans prepared in this way are useful reagents as the basis for glycan arrays, diagnostics, and carbohydrate-based vaccines.

• 出版日期2017-4-25