Duplex real-time PCR method for the detection of sesame (Sesamum indicum) and flaxseed (Linum usitatissimum) DNA in processed food products

作者：Lopez Calleja Ines Maria^*; de la Cruz Silvia; Martin Rosario; Gonzalez Isabel; Garcia Teresa

来源：Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure& Risk Assessment, 2015, 32(11): 1772-1785.

DOI：10.1080/19440049.2015.1079650

• duplex TaqMan real-time PCR
• sesame (Sesamum indicum)
• flaxseed (Linum usitatissimum)
• commercial food products
• food allergy

The development of a duplex real-time polymerase chain reaction (PCR) method allowing the simultaneous detection of sesame and flaxseed DNA in commercial food products is described. This duplex real-time PCR technique is based in the design of sesame- and flaxseed-specific primers based on the ITS1 region and two TaqMan fluorescent probes. The method was positive for sesame and flaxseed, and showed no cross-reactivity for all other heterologous plant and animal species tested. Sesame and flaxseed could be detected in a series of model samples with defined raw and heat-treated sesame in flaxseed, and flaxseed in sesame, respectively, with detection limits of 1.3mgkg(-1) for sesame and 1.4mgkg(-1) for flaxseed. The applicability of the assay for determining sesame and flaxseed in different food matrices was investigated by analysing a total of 238 commercial foodstuffs. This PCR method is useful for highly selective and sensitive detection of traces of sesame and flaxseed in commercial food products.

• 出版日期2015-11-2