Age-associated distribution of normal B-cell and plasma cell subsets in peripheral blood

作者：Blanco Elena; Perez Andres Martin; Arriba Mendez Sonia; Contreras Sanfeliciano Teresa; Criado Ignacio; Pelak Ondrej; Serra Caetano Ana; Romero Alfonso; Puig Noemi; Remesal Ana; Canizales Juan Torres; Lopez Granados Eduardo; Kalina Tomas; Sousa Ana E; van Zelm Menno; van der Burg Mirjam; van Dongen Jacques J M; Orfao Alberto^*

来源：Journal of Allergy and Clinical Immunology, 2018, 141(6): 2208-+.

DOI：10.1016/j.jaci.2018.02.017

• Immunoglobulins
• IgH isotype
• subclass
• memory B cells
• plasma cells
• flow cytometry
• reference ranges
• normal B cells
• age-related values

Background: Humoral immunocompetence develops stepwise throughout life and contributes to individual susceptibility to infection, immunodeficiency, autoimmunity, and neoplasia. Immunoglobulin heavy chain (IgH) isotype serum levels can partly explain such age-related differences, but their relationship with the IgH isotype distribution within memory Bcell (MBC) and plasma cell (PCs) compartments remains to be investigated. Objective: We studied the age-related distribution of MBCs and PCs expressing different IgH isotypes in addition to the immature/transitional and naive B-cell compartments. Methods: B-cell and PC subsets and plasma IgH isotype levels were studied in cord blood (n = 19) and peripheral blood (n = 215) from healthy donors aged 0 to 90 years by using flow cytometry and nephelometry, respectively. Results: IgH-switched MBCs expressing IgG(1), IgG(2), IgG(3), IgA(1), and IgA(2) were already detected in cord blood and newborns at very low counts, whereas CD27 1 IgM 11 IgD 1 MBCs only became detectable at 1 to 5 months and remained stable until 2 to 4 years, and IgD MBCs peaked at 2 to 4 years, with both populations decreasing thereafter. MBCs expressing IgH isotypes of the second immunoglobulin heavy chain constant region (IGHC) gene block (IgG(1), IgG(3), and IgA(1)) peaked later during childhood (2-4 years), whereas MBCs expressing third IGHC gene block immunoglobulin isotypes (IgG2, IgG4, and IgA2) reached maximum values during adulthood. PCs were already detected in newborns, increasing in number until 6 to 11 months for IgM, IgG(1), IgG(2), IgG(3), IgA(1), and IgA(2); until 2 to 4 years for IgD; and until 5 to 9 years for IgG(4) and decreasing thereafter. For most IgH isotypes (except IgD and IgG4), maximum plasma levels were reached after PC and MBC counts peaked. Conclusions: PC counts reach maximum values early in life, followed by MBC counts and plasma IgH isotypes. Importantly, IgH isotypes from different IGHC gene blocks show different patterns, probably reflecting consecutive cycles of IgH isotype switch recombination through life.

• 出版日期2018-6