Anti-inflammatory Effect of Glycoprotein Isolated from Cudrania Tricuspidata Bureau: Involvement of MAPK/NF-kappa B Signaling

作者：Oh Phil Sun; Lim Kye Taek^*

来源：Immunological Investigations, 2011, 40(1): 76-91.

DOI：10.3109/08820139.2010.516049

• Cudrania tricuspidata Bureau (CTB) glycoprotein
• Immunoglobulin E
• Interleukin-4

The aim of this study was to evaluate inhibitory effect of glycoprotein isolated from Cudrania tricuspidata Bureau (CTB glycoprotein) on allergic responses. We evaluated the activities of mitogen-activated protein kinase (MAPK), transcriptional factor, and production of immunoglobulin (Ig) E and interleukin (IL)-4 in RBL-2H3 cells and BALB/c mice. Our results showed that CTB glycoprotein inhibited the production of IgE and IL-4 in serum from ovalbumin (OVA)-treated BALB/c mice. We also found that CTB glycoprotein inhibited the phosphorylation of p38 MAPK, and transcriptional activation of nuclear factor (NF)-kappa B in RBL-2H3 cells. The activation of NF-kappa B was effectively blocked by treatment with p38 MAPK inhibitor (SKF86002). The results from these experiments indicate that the CTB glycoprotein inhibits release of beta-hexosaminidase, and production of IgE and IL-4 via down regulation of MAPK/NF-kappa B on the stage of mast cell degranulation. In conclusion, we suggest that the CTB glycoprotein might be a potent preventive agent in allergic responses.

• 出版日期2011