Analysis of Antigenic Determinant Glycans of Peanut Allergy Glycoprotein Ara h1 by Mass Spectrometry

作者：Peng Yifang; Wang Chengjian; Wang Jingjing; Li Lingmei; Jin Wanjun; Qiang Shan; Shi Hongdan; Zhang Ying; Huang Linjuan; Wang Zhongfu^*

来源：Chemical Journal of Chinese Universities, 2016, 37(9): 1622-1628.

DOI：10.7503/cjcu20160147

• Peanut allergy
• Allergenic glycoprotein Ara h1
• Glycan structure
• Electrospray ionization mass spectrometry (ESI-MS)
• Hydrophilic interaction liquid chsomatography-mass spectrometry (HILIC-MS)

Peanut seed total protein and glycoprotein Ara h1, which can cause allergic reactions, were chosen as the target materials in this study. Firstly, the glycans were released and labeled with 1-pheny1-3-methyl-5pyrazolone(PMP) by recently developed "one-pot" method. Subsequently, the glycans were fractionated and enriched through C18 solid phase extraction column. Finally, the obtained glycans were analysed by electrospray ionization mass spectrometry (ESI-MS), MS and Hydrophilic interaction liquid chsomatography-mass spectrometry (HILIC-MS). The results indicated that Ara h1 has ten N-glycans, including seven high-mannose type, two ones with xylose modification, and one allergenic core alpha 1;3-fucosylated N-glycan, the content of which is 12.45% of total glycans.

• 出版日期2016-9-10
• 单位西北大学