PAR-4 and hTERT expression are negatively correlated after RNA interference targeting hTERT in laryngocarcinoma cells

作者：Sheng, Jian-Fei; Chen, Wei; Yu, Yan; Liu, Jun; Tao, Ze-Zhang^*

来源：Tissue and Cell, 2010, 42(6): 365-369.

DOI：10.1016/j.tice.2010.08.002

• Human telomerase reverse transcriptase
• Prostate apoptosis response 4
• Larygocarcinoma
• Short hairpin RNA

We investigated the relationship between telomerase activity and the expression of human telomerase reverse transcriptase (hTERT) and prostate apoptosis response 4 (Par-4) proteins in laryngocarcinoma cells. Following treatment with short hairpin RNA (shRNA) against hTERT, hTERT protein expression was inhibited, while Par-4 expression was increased. These changes were statistically significant (p < 0.05), with Par-4 and hTERT expression being negatively correlated (p < 0.05, r = -0.908). However, we found no correlation between telomerase activity and hTERT expression. Our findings suggest that hTERT exerts its anti-apoptotic effects through a telomerase-independent pathway, which is likely to intersect with the Par-4 apoptosis pathway.

• 出版日期2010-12
• 单位武汉大学