Anti-high mobility group box 1 and box 2 non-histone chromosomal proteins (HMGB1/HMGB2) antibodies and anti-Saccharomyces cerevisiae antibodies (ASCA): accuracy in differentially diagnosing UC and CD and correlation with inflammatory bowel disease phenotype

作者：Takaishi Hiromasa; Kanai Takanori; Nakazawa Atsushi; Sugata Fumihiko; Nikai Akira; Yoshizawa Shigeo; Hamamoto Yasuo; Funakoshi Shinsuke; Yajima Tomoharu; Iwao Yasushi; Takemura Masao; Ozaki Shoichi; Hibi Toshifumi^*

来源：Journal of Gastroenterology, 2012, 47(9): 969-977.

DOI：10.1007/s00535-012-0566-3

• Anti-high mobility group box 1 and box 2 non-histone chromosomal proteins
• Anti-Saccharomyces cerevisiae antibodies
• Ulcerative colitis
• Crohn%26apos
• s disease

The development of a supportive diagnostic method has long been required to differentially diagnose ulcerative colitis (UC) and Crohn%26apos;s disease (CD). Several antibodies circulate in the sera of patients with inflammatory bowel disease. We previously identified the high mobility group box 1 and box 2 non-histone chromosomal proteins (HMGB1 and HMGB2) as novel antigens of perinuclear type anti-neutrophil cytoplasmic antibodies (pANCA) and discovered anti-HMGB1/HMGB2 antibodies in sera from patients with UC. Here, we evaluated the ability of anti-HMGB1/HMGB2 antibodies combined with anti-Saccharomyces cerevisiae antibodies (ASCA) to differentially diagnose UC and CD. %26lt;br%26gt;We measured titers of anti-HMGB1/HMGB2 antibodies and ASCA in the sera of 213 patients with UC and 93 with CD, using enzyme-linked immunosorbent assays. %26lt;br%26gt;Among the patients with UC, 26.8% were positive for anti-HMGB1/HMGB2 antibodies, with 85.0% specificity towards CD and a positive predictive value of 80.3%. Corticosteroids significantly suppressed the titer of anti-HMGB1/HMGB2 antibodies. Among the patients with CD, 24.7% were positive for ASCA, with 96.2% specificity towards UC and a positive predictive value of 74.2%. Interestingly, the positivity rate of anti-HMGB/HMGB2 antibodies was higher (35.7%) in patients with the ileitis type of CD than in patients with CD in the colon (6.2%; significant difference, P %26lt; 0.01). The specificity of anti-HMGB1/HMGB2 antibodies in UC for CD in the colon was 93.8%. %26lt;br%26gt;CD in the colon and UC can be differentially diagnosed using anti-HMGB/HMGB2 antibodies combined with ASCA.

• 出版日期2012-9