Binding modes of DL-2-haloacid dehalogenase revealed by crystallography, modeling and isotope effects studies

作者：Siwek Agata; Omi Rie; Hirotsu Ken; Jitsumori Keiji; Esaki Nobuyoshi; Kurihara Tatsuo^*; Paneth Piotr

来源：Archives of Biochemistry and Biophysics, 2013, 540(1-2): 26-32.

DOI：10.1016/j.abb.2013.09.012

• DL-DEX
• Dehalogenation
• Mechanism
• Chlorine isotope effect
• Binding isotope effect

Several pathways of biotic dechlorination can be found in enzymes, each characterized by different chlorine isotopic fractionation, which can thus serve as a signature of a particular mechanism. Unlike other dehalogenases, DL-2-haloacid dehalogenase, DL-DEX, converts both enantiomers of the substrate. Chlorine isotope effects for this enzyme are larger than in the case of other dehalogenases. Recently, the 3D structure of this enzyme became available and enabled us to model these isotope effects and seek their origin. We show that the elevated values of the chlorine kinetic isotope effects originate in part in the processes of binding and migration within the enzyme active site that precede the dehalogenation step.

• 出版日期2013-12